WO2008151324A1

WO2008151324A1 - Reduction processes for the preparation of ezetimibe

Info

Publication number: WO2008151324A1
Application number: PCT/US2008/066351
Authority: WO
Inventors: Nurit Perlman; Ayelet Fishman
Original assignee: Teva Pharmaceutical Industries Ltd.; Teva Pharmaceuticals Usa, Inc.
Priority date: 2007-06-07
Filing date: 2008-06-09
Publication date: 2008-12-11
Also published as: EP2155667A1

Abstract

Processes for preparing ezetimibe-related compounds with a ketoreductase and for purifying ezetimibe are disclosed.

Description

REDUCTION PROCESSES FOR THE PREPARATION OF EZETIMIBE

CROSS-REFERENCE TO RELATED APPLICATIONS

[001] This application claims the benefit of Provisional Application Serial No.

60/933,837, filed June 7, 2007, Provisional Application Serial No. 60/971,504, filed September 11, 2007; Provisional Application Serial No. 61/004,725, filed November 28, 2007, Provisional Application Serial No. 61/005,389, filed December 4, 2007, and Provisional Application Serial No. 61/050,875, filed May 6, 2008, each of which is incorporated herein by reference in its entirety.

FIELD OF THE INVENTION

[002] The invention relates to reduction processes of ezetimibe intermediates to obtain ezetimibe or a derivative thereof.

BACKGROUND OF THE INVENTION

[003] Hydroxy-alkyl substituted azetidinones are useful as hypercholesterolemia agents in the treatment and prevention of atherosclerosis. Ezetimibe, l-(4-fluorophenyl)- 3(R)-[3-(4-fluorophenyl)-3(S)-hydroxypropyl]-4(S)-(4-hydroxyphenyl)-2-azetidinone, is a selective inhibitor of intestinal cholesterol and related phytosterol absorption. The empirical formula for ezetimibe is C₂₄H₂1F2NO3, and its molecular weight is 409.4. Ezetimibe is a white, crystalline powder that is freely to very soluble in ethanol, methanol, and acetone and practically insoluble in water. Ezetimibe has the following chemical structure:

Ezetimibe

[004] Ezetimibe is the active ingredient in the drug sold under the brand name

ZETIA^®, which is manufactured by Merck/Schering-Plough Pharmaceuticals. ZETIA^® has been approved by the United States Food and Drug Administration for use in patients with high cholesterol to reduce low density lipoprotein ("LDL") cholesterol and total cholesterol.

[005] Ezetimibe can be prepared by reducing (3R,4S)-4-((4-benzyloxy)phenyl)-l-(4- fluorophenyl)-3-(3-(4-fluorophenyl)-3-oxopropyl)-2-azetidinone ("Compound 1") with borane dimethyl sulfide complex or borane tetrahydrofuran complex in tetrahydrofuran in the presence of Corey's reagent and subsequently deprotecting the benzyl group, as shown in Scheme 1 below. The process is disclosed in US patent nos. 5,631,365 ("the '365 patent") and 6,627 ',757 ', which are incorporated herein by reference. The starting material, Compound 1 or a similar compound, can be prepared by processes known in the art, for example, those disclosed in the '365 patent.

Scheme 1

Compound 1

Compound 2a (SRS isomer) Compound 2b (RRS isomer)

Ezetimibe

[006] The reduction process produces two isomers, (3R,4S)-4-((4- benzyloxy)phenyl)-l-(4-fluorophenyl)-3-((S)-3-(4-fluorophenyl)-3-hydroxypropyl)-2- azetidinone ("Compound 2a") and (3R,4S)-4-((4-benzyloxy)phenyl)-l-(4-fluorophenyl)-3- ((R)-3-(4-fluorophenyl)-3-hydroxypropyl)-2-azetidinone ("Compound 2b"). Compound 2a is the desired isomer that produces ezetimibe of the proper chirality. Compound 2b is an undesirable isomer that is very difficult to remove both during reduction as well as the final synthesis to form ezetimibe. It has been reported that Compound 2b is typically produced in about 8 to 10% yield during the reduction process.

[007] The '365 patent refers to the reduction of Compound 1 to Compound 2a by

(R)-(+)-2-methyl-CBS-oxazaborolidine ("CBS", Corey-Bakshi-Shibata catalyst) and borohydride dimethylsulfide complex ("BMS"), as illustrated below.

Compound 1 Compound 2a

[008] U.S. patent No. 6,133,001 refers to a process for stereoselective microbial reduction of ezetimibe-ketone to ezetimibe, as illustrated below.

Ezetimibe-ketone Ezetimibe

[009] PCT publication No. WO 2005/066120 refers to an enantioselective reduction of ezetimibe-ketone to ezetimibe with (-)-B-chlorodiisopinocampheylborane ("DIP-Cl").

[0010] There is a need for improved methods for preparing ezetimibe and derivatives thereof.

SUMMARY OF THE INVENTION

[0011] In one embodiment, the invention encompasses a process for preparing a compound of formula I

comprising combining a compound of formula II with an isolated, synthesized, or purified ketoreductase,

(II) wherein R is H or a hydroxyl protecting group. Preferably, the ketoreductase is selected from the group consisting of the predominant enzyme in each of KRED-NADH- 105, KRED- NADH-107, KRED-116, KRED-118, KRED-119, KRED-120, KRED-128, KRED-133, and mixtures thereof.

[0012] In one embodiment, the invention encompasses a process for preparing a compound of formula I

comprising combining a compound of formula II

a ketoreductase enzyme, a co-factor, and a buffer having a pH of about 4 to about 8, wherein R is H or a hydroxyl protecting group.

[0013] In one embodiment, the invention encompasses a process for purifying ezetimibe from EZT-ketone comprising crystallizing ezetimibe from MIBK.

DETAILED DESCRIPTION OF THE INVENTION

[0014] In one aspect, the present invention relates to a process for preparing a compound of formula I

using enzymes as catalysts and optionally involves the use of water-miscible organic solvents.

[0015] As used herein, the term "yield" refers to percentage of a synthesized compound with respect to the original amount of starting material. It is determined by the area percentage under the peak of the synthesized compound relative to the total area in the HPLC chromatogram. Yield is typically measured during or immediately following a reaction.

[0016] As used herein, the term "purity" refers to the percentage of a compound with respect to other compounds. It is determined by the area percentage under the peak of the compound relative to the total area in the HPLC chromatogram. Purity is typically measured after a purification process.

[0017] As used herein, the term "d.e." refers to diastereometric excess, defined as:

(mole fraction of ezetimibe) minus (mole fraction of a R,R,S-diastereomer of ezetimibe).

. _ (% Ezetimibe - % R,R,S-diastereomer)

(% Ezetimibe + % R,R,S-diastereomer).

Ezetimibe has a S,R,S configuration, as shown below:

Ezetimibe. The R,R,S-diastereomer differs in that it has an (R) configuration at the chiral center on the third carbon in the propyl chain.

[0018] As used herein, the term "EZT" refers to ezetimibe, or l-(4-fluorophenyl)-

3(R)-[3-(4-fluorophenyl)-3(S)-hydroxypropyl]-4(S)-(4-hydroxyphenyl)-2-azetidinone. As used herein, the term "EZT RRS-isomer" refers to l-(4-fluorophenyl)-3(R)-[3-(4- fluorophenyl)-3(R)-hydroxypropyl]-4(S)-(4-hydroxyphenyl)-2-azetidinone. As used herein, the term "ezetimibe-ketone" or "EZT-ketone" refers to l-(4-fluorophenyl)-3(R)-[3-(4- fluorophenyl)-3-oxopropyl]-4(S)-(4-hydroxyphenyl)-2-azetidinone, having the following chemical structure:

[0019] As used herein, the term "Compound 1 " refers to (3R,4S)-4-((4- benzyloxy)phenyl)- 1 -(4-fluorophenyl)-3-(3-(4-fluorophenyl)-3-oxopropyl)-2-azetidinone; the term "Compound 2a" refers to (3R,4S)-4-((4-benzyloxy)phenyl)-l-(4-fluorophenyl)-3-((S)-3- (4-fluorophenyl)-3-hydroxypropyl)-2-azetidinone; and the term "Compound 2b" refers to (3R,4S)-4-((4-benzyloxy)phenyl)-l-(4-fluorophenyl)-3-((R)-3-(4-fiuorophenyl)-3- hydroxypropyl)-2-azetidinone.

[0020] As used herein, when applied to an enzyme, the term "isolated" refers to an enzyme separated or removed from its native environment. Use of the term "isolated" indicates that a naturally occurring or recombinant enzyme has been removed from its normal cellular environment. Preferably, the isolated enzyme is in a cell-free system. The isolated enzyme can be crude or highly purified depending on the effort put in removing other materials. The term "isolated" does not imply that the enzyme is the only enzyme present, but that it is the predominant enzyme present (at least 10-20% more than any other enzyme). As used herein, when applied to an enzyme, the term "synthesized" refers to an enzyme that is prepared by chemical synthesis, recombinant means, or the combination thereof. As used herein, when applied to an enzyme, the term "purified" refers to an enzyme that is essentially free (at least about 90-95% pure) of non-enzymatic material or other enzymes.

[0021] As used herein, the term "dehydrogenase" or "dehydrogenase enzyme" refers to an enzyme that oxidizes a substrate by transferring one or more protons and a pair of electrons to an acceptor. Non- limiting examples of dehydrogenase include alcohol dehydrogenase, glucose dehydrogenase, formate dehydrogenase, and phosphite dehydrogenase. Glucose dehydrogenase ("GDH") include, for example, those classified under the Enzyme Commission ("EC") number of 1.1.1.47 and the Chemical Abstract Service ("CAS") number 9028-53-9, and are commercially available, for example, from Codexis, Inc. (formerly BioCatalytics, Inc) under the catalog numbers GDH- 102 to GDH- 104. Formate dehydrogenase ("FDH") include, for example, those classified under the EC number of 1.2.1.2 and the CAS number of 9028-85-7, and are commercially available, for example, from Codexis, Inc. under the catalog number FDH-101. Phosphite dehydrogenase ("PDH") include, for example, those classified under the EC number of 1.20.1.1 and CAS number of 9031-35-0, and are commercially available, for example, from Codexis, Inc. under the catalog number PDH-101.

[0022] As used herein, the term "ketoreductase," "ketoreductase enzyme," or

"KRED" refers to an enzyme that catalyzes the reduction of a ketone to form the corresponding alcohol. Ketoreductase enzymes include, for example, those classified under the EC numbers of 1.1.1. Such enzymes are given various names in addition to ketoreductase, including, but not limited to, alcohol dehydrogenase, carbonyl reductase, lactate dehydrogenase, hydroxyacid dehydrogenase, hydroxyisocaproate dehydrogenase, β- hydroxybutyrate dehydrogenase, steroid dehydrogenase, sorbitol dehydrogenase, aldoreductase, and the like. NADPH-dependent ketoreductases are classified under the EC number of 1.1.1.2 and the CAS number of 9028-12-0. NADH-dependent ketoreductases are classified under the EC number of 1.1.1.1 and the CAS number of 9031 -72-5. Ketoreductases are commercially available, for example, from Codexis, Inc. under the catalog numbers KRED- 100 to KRED- 177.

[0023] As used herein, the term "co-factor" refers to a non-protein compound that operates in combination with an enzyme which catalyzes the reaction of interest. Co-factors include, for example, nicotinamide co-factors such as nicotinamide adenine dinucleotide ('TSfAD"), reduced nicotinamide adenine dinucleotide ("NADH"), nicotinamide adenine dinucleotide phosphate ("NADP⁺"), reduced nicotinamide adenine dinucleotide phosphate ("NADPH"), and any derivatives or analogs thereof.

[0024] As used herein, the term "MeOH" refers to methanol; the term "EtOAc" refers to ethyl acetate; the term "IPA" refers to isopropyl alcohol; the term "DMSO" refers to dimethyl sulfoxide"; the term "MIBK" refers to methyl isobutyl ketone ; the term "DCM" refers to dichlormethane; the term "MTBE" refers to methyl tert-butyl ether, and the term "DTT" refers to dithiotreitol.

[0025] As used herein, the term "room temperature" or "RT" refers the ambient temperature of a typical laboratory, which is usually about 15⁰C to about 3O⁰C.

[0026] As used herein, the term "vacuum" refers to a pressure of about to 2 mmHg to about 100 mmHg.

[0027] In one embodiment, the invention encompasses a process for preparing a compound of formula I

wherein R is H or a hydroxyl protecting group. Preferably, the ketoreductase is selected from the group consisting of the predominant enzyme in each of KRED-NADH- 105, KRED- NADH-107, KRED-116, KRED-118, KRED-119, KRED-120, KRED-128, KRED-133, and mixtures thereof.

[0028] In one embodiment, the invention encompasses a process for preparing a compound of formula I

comprising combining a compound of formula II

a biocatalyst, and a buffer, wherein R is H or a hydroxyl protecting group.

[0029] In one embodiment, the invention encompasses a process for preparing a compound of formula I

comprising combining a compound of formula II

a ketoreductase enzyme, a co-factor, and a buffer having a pH of about 4 to about 8, wherein R is H or a hydroxy 1 protecting group.

[0030] When R is H, the compound of formula I is ezetimibe and the compound of formula II is ezetimibe-ketone. When R is a hydroxyl protecting group, the compound of formula II can be further deprotected to obtain ezetimibe. The deprotection can be done by known methods, for example those described in Example 6 of the '365 patent.

[0031] The reaction is an enzymatic process as illustrated below:

Ketoreductase NAD(P)H ^enzyme

In this reaction, the ketoreductase stereoselectively reduces the carbonyl group. At the same time, a co-factor such as NADH or NADPH is oxidized.

[0032] Preferably, the hydroxyl protecting group is selected from the group consisting of benzyl, tert-butyloxycarbonyl, acyl, and silyl groups. Examples of suitable silyl protecting groups include, but are not limited to, (R^a)(R^b)(R^c)-Si-, wherein R^a, R^b and R^c are each independently selected from the group consisting of Ci to C₆ alkyl, phenyl, benzyl, acetyl, or the like.

[0033] Preferably, the ketoreductase is isolated. The ketoreductase can be separated from any host, such as mammals, filamentous fungi, yeasts, and bacteria. The isolation, purification, and characterization of a NADH-dependent ketoreductase is described in, for example, in Kosjek et al, Purification and Characterization of a Chemotolerant Alcohol Dehydrogenase Applicable to Coupled Redox Reactions, Biotechnology and Bioengineering, 86:55-62 (2004). Preferably, the ketoreductase is synthesized. The ketoreductase can be synthesized chemically or using recombinant means. The chemical and recombinant production of ketoreductases is described in, for example, in European patent no. EP 0918090. Preferably, the ketoreductase is synthesized using recombinant means in Escherichia coli. Preferably, the ketoreductase is purified, preferably with a purity of about 90% or more, more preferably with a purity of about 95% or more. Preferably, the ketoreductase is substantially cell-free.

[0034] Preferably, the ketoreductase is one that capable of producing ezetimibe with a d.e. of about 90% or higher in the processes of the invention. Preferably, the ketoreductase is one that capable of producing ezetimibe with a yield of about 50% or higher in the processes of the invention.

[0035] Preferably, the ketoreductase is selected from the group consisting of NADH- dependent ketoreductases and NADPH-dependent ketoreductases. Suitable ketoreductases include, but are not limited to, a) Codexis Inc's products with catalog numbers KRED- NADH- 105, KRED-NADH- 107, KRED-116, KRED-118, KRED-119, KRED- 120, KRED- 128, KRED-133, equivalent products thereof, and mixtures thereof; and b) the predominant enzyme in each of Codexis Inc's products with catalog numbers KRED-NADH- 105, KRED- NADH-107, KRED-116, KRED-118, KRED-119, KRED-120, KRED-128, KRED-133, equivalent enzymes thereof, and mixtures thereof. As used herein, the term "equivalent" refers to an enzyme or product with similar or identical enzymatic activity. Preferably, the ketoreductase is selected from the group consisting of the predominant enzyme in each of Codexis Inc's products with catalog numbers KRED-NADH- 105, KRED-NADH- 107, KRED-116, KRED-118, KRED-119, KRED-120, KRED-128, KRED-133, and mixtures thereof. Preferably, the ketoreductase is selected from the group consisting the predominant enzyme in each of KRED-NADH- 105, KRED-116, KRED-118, KRED-119, KRED-128, and mixtures thereof. More preferably, the ketoreductase is selected from the group consisting of the predominant enzyme in each of KRED-118, KRED-119, KRED-128, and mixtures thereof.

[0036] In one embodiment, the process of the invention further comprises combining a co-factor with the ketoreductase. Preferably, the co-factor is selected from the group consisting of NADH, NADPH, NAD⁺, NADP⁺, salts thereof, and mixtures thereof. Preferably, when the ketoreductase is NADH-dependent, the co-factor is selected from the group consisting of NADH, NAD⁺, salts thereof, and mixtures thereof. More preferably, the co-factor is NADH or a salt thereof. Preferably, when the ketoreductase is NADPH- dependent, the co-factor is selected from the group consisting of NADPH, NADP⁺, salts thereof, and mixtures thereof. More preferably, the co-factor is NADPH or a salt thereof.

[0037] In one embodiment, the process of the invention is carried out in a buffer.

Preferably, the buffer has a pH of from about 4 to about 9, more preferably from about 4 to about 8, more preferably from about 5 to about 8, most preferably from about 6 to about 8 or about 5 to about 7. Preferably, the buffer is a solution of a salt. Preferably, the salt is selected from the group consisting of potassium phosphate, magnesium sulfate, and mixtures thereof. Optionally, the buffer comprises a thiol. Preferably, the thiol is DTT. Preferably, the thiol reduces the disulfide bond in the enzyme.

[0038] In one embodiment, the process of the invention is carried out at a temperature of about 1O⁰C to about 5O⁰C. Preferably, the process is carried out at room temperature, at a temperature of about 24⁰C to about 28⁰C, or at about 25⁰C to about 35⁰C. Preferably, the process is carried out at a temperature of about 25⁰C to about 3O⁰C. Preferably, the process is carried out at a temperature of about 3O⁰C.

[0039] Optionally, the reaction mixture further comprises a co-factor regeneration system. A co-factor regeneration system comprises a substrate and a dehydrogenase. The reaction between the substrate and dehydrogenase enzyme regenerates the co-factor. Preferably, the co-factor regeneration system comprises a substrate/dehydrogenase pair selected from the group consisting of D-glucose/glucose dehydrogenase, sodium formate/formate dehydrogenase, and phosphite/phosphite dehydrogenase. Preferably, the glucose dehydrogenase is selected from the group consisting of the predominant enzyme in each of Codexis Inc's products with catalog numbers GDH-102, GDH-103, GDH-104, and mixtures thereof. Preferably, the glucose dehydrogenase is the enzyme in GDH-104. Preferably, the formate dehydrogenase is the predominant enzyme in Codexis Inc's product with catalog number FDH-101. Preferably, the phosphite dehydrogenase is the predominant enzyme in Codexis Inc's product with catalog number PDH-101.

[0040] In one embodiment, the process of the invention further comprises adding a solvent. Preferably, the solvent is an organic solvent. Preferably, the organic solvent is water-miscible. Preferably the water-miscible organic solvent is selected from the group consisting of alcohols and DMSO. Preferably, the alcohol is a C1-C4 alcohol, more preferably methanol or IPA. The advantage of the preferred solvents used in this process, compared to the organic solvents used in prior art references, is that their medium is mostly water, which makes the reaction more environmentally friendly.

[0041] Preferably, the process comprises the following steps: (a) dissolving a compound of formula II in a solvent; and (b) combining the solution from (a) with a buffer containing a co-factor and a ketoreductase. Preferably, the obtained mixture is stirred for a period of time sufficient to obtain the compound of formula I. Preferably, the stirring is at a temperature of about 25⁰C to about 35⁰C or about 25⁰C to about 4O⁰C, more preferably at a temperature of about 24⁰C to about 28⁰C, about 25⁰C to about 3O⁰C, or about 3O⁰C. Preferably, the stirring is for about 0.5 hours or more, about 1.5 hours or more, or about 2.5 hours or more. Preferably, the stirring is for about 50 hours or less. Preferably, the stirring is for about 3 hours to about 40 hours, more preferably for about 6 hours to about 24 hours or about 6 hours to about 16 hours.

[0042] Optionally, a water-immiscible organic solvent is added to the reaction mixture, preferably after the stirring. Optionally, after the water-immiscible organic solvent is added, the reaction mixture is separated into an organic phase and an aqueous phase. Optionally, the compound of formula I is recovered by evaporating the organic phase. Examples of water-immiscible organic solvents include, but are not limited to, C₂-Cs ethers, C₃-Cs esters such as EtOAc, C₄-Cs ketones such as MIBK, and halogenated hydrocarbons such as DCM. Preferably, the water-immiscible organic solvent is selected from the group consisting of EtOAc, MTBE, diethyl ether, and mixtures tehreof. Preferably, the water- immiscible organic solvent is EtOAc.

[0043] Optionally, the reaction mixture, preferably after the stirring, is filtered to recover the solid product, which may optionally be further purified to obtain the compound of formula I.

[0044] Optionally, after the product is separated, the aqueous phase may be treated to recycle the enzyme, co-factor, and/or the dehydrogenase in the co-factor regeneration system. Optionally, the pH of the aqueous phase may be adjusted to obtain the desired pH. Optionally, the aqueous phase is evaporated to remove organic solvent residue. Optionally, the aqueous phase is reused in the process of the invention. [0045] Preferably, the processes described above have high stereoselectivity toward ezetimibe. The d.e. of the ezetimibe obtained is preferably about 90% or higher, more preferably about 98% or higher, or about 99% or higher, and most preferably about 99.9% or higher.

[0046] Preferably, the processes described above has high yield. The yield of the compound of formula I obtained is preferably about 50% or higher, more preferably about 60% or higher, or about 70% or higher, or about 80% or higher and most preferably about 85% or higher.

[0047] In one embodiment, the invention encompasses a process for purifying ezetimibe from EZT-ketone comprising crystallizing ezetimibe from MIBK. Preferably, the process comprises: a) dissolving a sample comprising ezetimibe and EZT-ketone in MIBK; b) cooling the solution from step a); and c) recovering ezetimibe.

[0048] Preferably, step a) is performed under heating. Preferably, the heating is to a temperature of from about 5O⁰C to about reflux temperature, more preferably to about reflux temperature. Optionally, the cooling is to about room temperature or less, preferably to about 1O⁰C. Optionally, a slurry is obtained after the cooling step. Optionally, ezetimibe is recovered from the slurry by filtering and optionally drying. The drying is preferably at about 4O⁰C to about 5O⁰C, preferably under vacuum.

[0049] Preferably, the ezetimibe obtained has a purity of about 98% or more, more preferably about 99% or more.

[0050] Having thus described the invention with reference to particular preferred embodiments and illustrative examples, those in the art can appreciate modifications to the invention as described and illustrated that do not depart from the spirit and scope of the invention as disclosed in the specification. The Examples are set forth to aid in understanding the invention but are not intended to, and should not be construed to, limit its scope in any way. Absent statement to the contrary, any combination of the specific embodiments described above are consistent with and encompassed by the present invention.

EXAMPLES

HPLC Method for Determination of Impurity Profile of Ezetimibe Column & Packing: Hypersil GOLD™,C8, 150*4.6 mm, 3μm, 25203-154630;

^Buffer: 0.05% TFA (trifluoracetic acid) Eluent A; 51 : 49 , Methanol : Buffer

Eluent B: 75 : 25 , Acetonitrile : Eluent A

Time % Eluent A % Eluent B

0 100 0

Gradient: 18 100 0

35 0 100

35.1 100 0

Equilibrium time: 7 min

Sample volume: 5 μL

Flow Rate: 1.5 mL/min

Detector: 235 nm

Column temperature: 3O ⁰C

Autosampler temperature: 1O⁰C

Diluent: methanol

Typical Retention Times

Example 1. Reduction of EZT-ketone with KRED- 128

[0051] KRED-128 (5mg, Codexis, Lot No. 090305CL, year of production: 2005) was dissolved in 5ml buffer (containing 25OmM potassium phosphate, 0.5mM DTT, 2mM magnesium sulfate, l.lmM NADP⁺, 8OmM D-glucose, 10 U/ml glucose dehydrogenase, pH 7.0). A solution of EZT-ketone in MeOH (lOmg in 0.25ml) was added. The mixture was stirred at 30⁰C for 40 hours and monitored by HPLC. EtOAc (5ml) was added and the phases were separated. The EZT in the organic phase was analyzed, (yield: 71.55%, d.e.: 99.9%).

Example 2. Reduction of EZT-ketone with KRED- 133

[0052] KRED- 133 (5mg, Codexis, Lot No. 113006MM, year of production: 2006) was dissolved in 5ml buffer (containing 25OmM potassium phosphate, 0.5mM DTT, 2mM magnesium sulfate, l.lmM NADP⁺, 8OmM D-glucose, 10 U/ml glucose dehydrogenase, pH 7.0). A solution of EZT-ketone in MeOH (lOmg in 0.25ml) was added. The mixture was stirred at 30⁰C for 40 hours and monitored by HPLC. EtOAc (5ml) was added and the phases were separated. The EZT in the organic phase was analyzed, (yield: 17.6%, d.e.: 99.3%).

Example 3. Reduction of EZT-ketone with KRED-NADH- 105

[0053] KRED-NADH -105 (5mg, Codexis) was dissolved in 5ml buffer (containing

25OmM potassium phosphate, 0.5mM DTT, 2mM magnesium sulfate, 1.3mM NAD⁺, 8OmM D-glucose, 10 U/ml glucose dehydrogenase, pH 7.0). A solution of EZT-ketone in MeOH (20mg in 0.25ml) was added. The mixture was stirred at 30⁰C over night and monitored by HPLC. EtOAc (5ml) was added and the phases were separated. The EZT in the organic phase was analyzed, (yield: 57.11%, d.e.: 90.7%).

Example 4. Reduction of EZT-ketone with KRED-NADH- 107

[0054] KRED-NADH -107 (5mg, Codexis) was dissolved in 5ml buffer (containing

25OmM potassium phosphate, 0.5mM DTT, 2mM magnesium sulfate, 1.3mM NAD⁺, 8OmM D-glucose, 10 U/ml glucose dehydrogenase, pH 7.0). A solution of EZT-ketone in MeOH (20mg in 0.25ml) was added and the mixture was stirred at 30⁰C over night and monitored by HPLC. EtOAc (5ml) was added and the phases were separated. The EZT in the organic phase was analyzed, (yield: 16.6 %, d.e.: 98.5%).

Example 5. Reduction of EZT-ketone with KRED-116

[0055] KRED-116 (5mg, Codexis) was dissolved in 5ml buffer (contains 25OmM potassium phosphate, 0.5mM "DTT, 2mM magnesium sulfate, 1.ImM NADP⁺, 8OmM D- glucose, 10 U/ml glucose dehydrogenase, pH 7.0). A solution of EZT-ketone in MeOH (20mg in 0.25ml) was added and the mixture was stirred at 30⁰C over night. EtOAc (5ml) was added and the phases were separated. The organic phase was evaporated. The EZT in the residue was analyzed, (yield: 57.97 %, d.e.: 99.9%).

Example 6. Reduction of EZT-ketone with KRED-118

[0056] KRED- 118 (5mg, Codexis) was dissolved in 5ml buffer (contains 25OmM potassium phosphate, 0.5mM DTT, 2mM magnesium sulfate, l.lmM NADP⁺, 8OmM D- glucose, 10 U/ml glucose dehydrogenase, pH 7.0). A solution of EZT-ketone in MeOH (20mg in 0.25ml) was added and the mixture was stirred at 30⁰C over night. EtOAc (5ml) was added and the phases were separated. The organic phase was evaporated, (yield: 87.59%, d.e.: 99.9%). Example 7. Reduction of EZT-ketone with KRED-119

[0057] KRED-119 (5mg, Codexis, Lot No. 100407WW, year of production: 2007) was dissolved in 5ml buffer (contains 25OmM potassium phosphate, 0.5mM DTT, 2mM magnesium sulfate, l.lmM NADP⁺, 8OmM D-glucose, 10 U/ml glucose dehydrogenase, pH 7.0). A solution of EZT-ketone in MeOH (20mg in 0.25ml) was added and the mixture was stirred at 30⁰C over night. EtOAc (5ml) was added and the phases were separated. The organic phase was evaporated. The EZT in the residue was analyzed, (yield: 83.19%, d.e.: 99.9%).

Example 8. Reduction of EZT-ketone with KRED- 120

[0058] KRED-120 (5mg, Codexis) was dissolved in 5ml buffer (contains 25OmM potassium phosphate, 0.5mM DTT, 2mM magnesium sulfate, l.lmM NADP⁺, 8OmM D- glucose, 10 U/ml glucose dehydrogenase, pH 7.0). A solution of EZT-ketone in MeOH (20mg in 0.25ml) was added and the mixture was stirred at 30⁰C over night. EtOAc (5ml) was added and the phases were separated. The organic phase was evaporated. The EZT in the residue was analyzed, (yield: 37.41%, d.e.: 99.9%).

Example 9. Reduction of EZT-ketone with KRED-128

[0059] The solution of lOmg EZT-ketone in 0.25 ml MeOH was added to the following solution: 5mg KRED-128 (Codexis, Lot No. 090305CL, year of production: 2005), 36mg (4OmM) D-glucose, 4mg (ImM) NADP⁺, 2.5mg glucose dehydrogenase in 5ml phosphate buffer (pH 7). The obtained milky mixture was stirred at 35°C for 1.5 hours. The mixture was filteed, and the filtered material was analyzed (yield: 89.26%, d.e.: 99.9%).

Example 10. Reduction of EZT-ketone with KRED-119

[0060] A solution of 600mg EZT-ketone in 4ml IPA was added to 20 ml 10OmM phosphate buffer solution (pH 6) containing: 800mg (0.25M) D-glucose, 40mg glucose dehydrogenase, 16mg (ImM) NADP⁺, and 20mg KRED-119 (Codexis, Lot No. 100407WW, year of production: 2007. The obtained milky mixture was stirred at room temperature for

2.5 hours. (The reaction was monitored by HPLC until 74.4% conversion was observed).

The mixture was filtered to obtain 0.59g of wet product, (yield: 65.43%*).

* The % conversion is lower than 74.4%, possibly because the reaction is reversible.

Example 11. Purification of ezetimibe [0061] 2g of mixture of ezetimibe and EZT-ketone (purity of ezetimibe: 82%; EZT- ketone content: 15.8%) was dissolved in 5ml MIBK at reflux temperature. The solution was cooled to room temperature and stirred overnight. The obtained slurry was filtered and dried to obtain 0.9g of white product (purity of ezetimibe: 99%).

Claims

What is claimed is:

1. A process for preparing a compound of formula I

wherein R is H or a hydroxy 1 protecting group.

2. The process of claim 1, wherein the ketoreductase is isolated. 3. The process of any of claims 1-2, wherein the ketoreductase is synthesized. 4. The process of any of claims 1-3, wherein the ketoreductase is purified. 5. The process of any of claims 1-4, wherein R is hydrogen. 6. The process of any of claims 1-4, wherein R is a hydroxyl protecting group. 7. The process of claim 6, wherein R is a selected from the group consisting of benzyl, tert-butyloxycarbonyl, acyl, and silyl groups.

The process of claim 7, wherein the silyl group is (R^a)(R^b)(R^c)-Si-, wherein R^a, R^b and R^c are each independently selected from the group consisting of Ci to C₆ alkyl, phenyl, acetyl, and benzyl groups.

9. The process of any of claims 1-8, wherein the ketoreductase is selected from the group consisting of the predominant enzyme in each of KRED-NADH- 105, KRED- NADH-107, KRED-116, KRED-118, KRED-119, KRED-120, KRED-128, KRED- 133, and mixtures thereof.

10. The process of any of claim 9, wherein the ketoreductase is selected from the group consisting of the predominant enzyme in each of KRED-NADH- 105, KRED-116, KRED-118, KRED-119, KRED-128, and mixtures thereof.

11. The process of claim 10, wherein the ketoreductase is selected from the group consisting of the predominant enzyme in each of KRED-118, KRED-119, KRED- 128, and mixtures thereof.

12. The process of any of claims 1-11, further comprises combining a co-factor with the ketoreductase, wherein the co-factor is selected from the group consisting of NADH, NADPH, NAD⁺, NADP⁺, salts thereof, and mixtures thereof.

13. The process of claim 12, wherein the co-factor is NADH or a salt thereof.

14. The process of claim 12, wherein the co-factor is NADPH or a salt thereof.

15. The process of any of claims 1-14, wherein the process is carried out in a buffer having a pH of about 4 to about 9.

16. The process of claim 15, wherein the buffer has a pH of about 4 to about 8.

17. The process of claim 16, wherein the buffer has a pH of about 6 to about 8.

18. The process of any of claims 15-17, wherein the buffer is a solution of at least one salt selected from the group consisting of potassium phosphate, magnesium sulfate.

19. The process of any of claims 15-18, wherein the buffer comprises dithiotreitol.

20. The process of any of claims 1-19, wherein the process is carried out at a temperature of about 1O⁰C to about 5O⁰C.

21. The process of claim 20, wherein the process is carried out at a temperature of about 25⁰C to about 35⁰C.

22. The process of claim 21 , wherein the process is carried out at a temperature of about 25⁰C to about 3O⁰C.

23. The process of any of claims 1-22, wherein the reaction mixture further comprises s a co-factor regeneration system.

24. The process of claim 23, wherein the co-factor regeneration system comprises a substrate/dehydrogenase pair selected from the group consisting of D-glucose/glucose dehydrogenase, sodium formate/formate dehydrogenase, and phosphite/phosphite dehydrogenase.

25. The process of claim 24, wherein the substrate/dehydrogenase pair is D- glucose/glucose dehydrogenase.

26. The process of claim 25, wherein the glucose dehydrogenase is selected from the group consisting of the predominant enzyme in each of GDH- 102, GDH- 103, GDH- 104, and mixtures thereof.

27. The process of claim 26, wherein the glucose dehydrogenase is the enzyme in GDH- 104.

28. The process of claim 24, wherein the substrate/dehydrogenase pair is sodium formate/formate dehydrogenase.

29. The process of claim 28, wherein the formate dehydrogenase is the predominant enzyme in FDH- 101.

30. The process of claim 24, wherein the substrate/dehydrogenase pair is sodium phosphite/phosphite dehydrogenase.

31. The process of claim 30, wherein the phosphite dehydrogenase is the predominant enzyme in PDH- 101.

32. The process of any of claims 1-31, further comprising adding a solvent.

33. The process of claim 32, wherein the solvent is a water-miscible organic solvent.

34. The process of claim 33, wherein the solvent is selected from the group consisting of alcohols and dimethyl sulfoxide.

35. The process of claim 34, wherein the alcohol is a C1-C4 alcohol.

36. The process of claim 35, wherein the alcohol is methanol or isopropyl alcohol.

37. The process of any of claims 32-36, comprising:

(a) dissolving the compound of formula II in a solvent; (b) combining the solution from (a) with a buffer containing a co-factor and a ketoreductase.

38. The process of any of claims 1-37, wherein the reaction mixture is stirred for about 3 hours to about 40 hours.

39. The process of claim 38, wherein the reaction mixture is stirred for about 14 hours to about 24 hours.

40. The process of any of claims 38-39, wherein the reaction mixture is stirred at a temperature of about 25⁰C to about 35⁰C.

41. The process of any of claims 1-40, further comprising recovering the product by filtering the reaction mixture.

42. The process of any of claims 1-41, wherein a water immiscible organic solvent is added to the reaction mixture

43. The process of claim 42, wherein the water immiscible organic solvent is added to the reaction mixture after stirring.

44. The process of any of claims 42-43, wherein the reaction mixture is separated into an organic phase and an aqueous phase after the water immiscible organic solvent is added.

45. The process of any of claims 42-44, wherein the water immiscible organic solvent is selected from the group consisting OfC₂-Cs ethers, C₃-Cs esters, C₄-Cs ketones, halogenated hydrocarbons, and mixtures thereof.

46. The process of any of claims 44-45, further comprising recovering the product by evaporating the organic phase.

47. The process of any of claims 1-46, wherein the yield of the compound of formula I obtained is about 50% or higher.

48. The process of claim 47, wherein the yield is about 60% or higher.

49. The process of claim 48, wherein the yield is about 70% or higher.

50. The process of claim 49, wherein the yield is about 80% or higher.

51. The process of claim 50, wherein the yield is about 85% or higher.

52. The process of any of claims 1-5 and 9-51, wherein the compound of formula I is ezetimibe.

53. The process of claim 52, wherein the diastereometric excess of ezetimibe is about 90% or higher.

54. The process of claim 53, wherein the diastereometric excess of ezetimibe is about 98% or higher.

55. The process of claim 54, wherein the diastereometric excess of ezetimibe is about 99% or higher.

56. The process of claim 55, wherein the diastereometric excess of ezetimibe is about 99.9% or higher.

57. The ezetimibe produced by the process of any of claims 1 -56,

58. A process for purifying ezetimibe from EZT-ketone crystallizing ezetimibe from methyl isobutyl ketone.

59. The process of claim 58, comprising: a) dissolving a sample comprising ezetimibe and EZT-ketone in methyl isobutyl ketone; b) cooling the solution from step a); and c) recovering ezetimibe.

60. The process of claim 59, wherein step a) is performed under heating.

61. The process of claim 60, wherein the heating is to a temperature of from about 5O⁰C to about reflux temperature.

62. The process of claim 61, wherein the heating is to about reflux temperature.

63. The process of any of claims 59-62, wherein the cooling is to about room temperature or less.

64. The process of claim 63, wherein the cooling is to about 1O⁰C.

65. The process of any of claims 59-64, wherein a slurry is obtained after the cooling step, and wherein ezetimibe is recovered from the slurry by filtering.

66. The process of any of claims 58-65, wherein the obtained ezetimibe has a purity of about 98% or more.

67. The process of claim 66, wherein the obtained ezetimibe has a purity of about 99% or more.

68. The ezetimibe obtained by the process of any of claims 58-67.