WO2006091094A3 - Hemoglobin overexpression in fungal fermentations - Google Patents
Hemoglobin overexpression in fungal fermentations Download PDFInfo
- Publication number
- WO2006091094A3 WO2006091094A3 PCT/NL2006/050006 NL2006050006W WO2006091094A3 WO 2006091094 A3 WO2006091094 A3 WO 2006091094A3 NL 2006050006 W NL2006050006 W NL 2006050006W WO 2006091094 A3 WO2006091094 A3 WO 2006091094A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- oxygen
- fungal
- host cells
- hemoglobin
- proteins
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
- C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
- C12N9/0061—Laccase (1.10.3.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
- C12N9/242—Fungal source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The present invention relates to fungal host cells that are transformed with a nucleic acid construct encoding a fungal oxygen-binding proteins or fragments thereof that comprise the oxygen-binding domain. Upon transformation of the host cell with the construct, the oxygen-binding protein confers to the host cell improved fermentation characteristics as compared to untransformed host cells. These characteristics include e.g. increases in oxygen uptake rates, biomass densities, volumetric productivities and/or product yields. The invention further relates to fermentation processes in which the host cells are used and to fungal oxygen binding proteins, in particular fungal flavohemoglobins and hemoglobin domains, and to nucleotide sequences encoding these proteins.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11/795,043 US20080193969A1 (en) | 2005-01-12 | 2006-01-12 | Hemoglobin Overexpression in Fungal Fermentations |
EP06700803A EP1841872A2 (en) | 2005-01-12 | 2006-01-12 | Hemoglobin overexpression in fungal fermentations |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP05075097.5 | 2005-01-12 | ||
EP05075097 | 2005-01-12 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2006091094A2 WO2006091094A2 (en) | 2006-08-31 |
WO2006091094A3 true WO2006091094A3 (en) | 2007-07-12 |
Family
ID=34937994
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/NL2006/050006 WO2006091094A2 (en) | 2005-01-12 | 2006-01-12 | Hemoglobin overexpression in fungal fermentations |
Country Status (3)
Country | Link |
---|---|
US (1) | US20080193969A1 (en) |
EP (1) | EP1841872A2 (en) |
WO (1) | WO2006091094A2 (en) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102382863B (en) * | 2010-09-03 | 2015-04-22 | 中国科学院青岛生物能源与过程研究所 | Method for improving exocytosis capacity of engineering bacteria fatty acid |
CN103314098A (en) * | 2010-10-28 | 2013-09-18 | 道达尔炼油与销售部 | Use of monascus in organic acid production |
RS60259B1 (en) * | 2013-09-11 | 2020-06-30 | Impossible Foods Inc | Secretion of heme-containing polypeptides |
CN104140933B (en) * | 2014-08-06 | 2016-06-08 | 浙江省农业科学院 | Aspergillus terreus ZRV2011F5 and application thereof |
CN110029068B (en) * | 2019-04-10 | 2022-03-25 | 天津科技大学 | Aspergillus niger genetic engineering strain for high yield of organic acid under low dissolved oxygen condition and application thereof |
CN110863005A (en) * | 2019-12-30 | 2020-03-06 | 河南省科学院生物研究所有限责任公司 | Method for improving α -galactosidase constitutive expression quantity in pichia pastoris engineering bacteria through co-expression of hemoglobin |
CN115433738A (en) * | 2022-06-28 | 2022-12-06 | 深圳技术大学 | Recombinant aspergillus oryzae strain capable of enriching iron and construction method and application thereof |
CN115820746B (en) * | 2022-11-08 | 2023-08-18 | 华南理工大学 | Application of kinase gene in regulation and control of mycelial morphology of filamentous fungi |
CN115948427A (en) * | 2023-01-10 | 2023-04-11 | 中国海洋大学 | Oxymectin FHb, recombinant bacterium X33-pPICZ alpha A-102C300C-FHb and application thereof |
CN115960920B (en) * | 2023-01-10 | 2023-10-24 | 中国海洋大学 | Coroxepin FHb, recombinant bacterium X33-pPICZ alpha A-102C300C-FHb2 and application thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003250590A (en) * | 2001-12-27 | 2003-09-09 | National Institute Of Advanced Industrial & Technology | Aspergillus oryzae flh2 gene |
EP1384782A1 (en) * | 2001-03-30 | 2004-01-28 | National Institute of Advanced Industrial Science and Technology | Method of detecting the expression of aspergillus gene |
EP1609867A1 (en) * | 2004-06-25 | 2005-12-28 | Feng Chia University | Nucleic acid construct, expression vector and method for enhancing the production of recombinant protein |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5908764A (en) * | 1997-05-22 | 1999-06-01 | Solidago Ag | Methods and compositions for increasing production of erythromycin |
US7163819B2 (en) * | 2005-04-14 | 2007-01-16 | E. I. Du Pont De Nemours And Company | Bacterial hemoglobin genes and their use to increase carotenoid production |
-
2006
- 2006-01-12 EP EP06700803A patent/EP1841872A2/en not_active Ceased
- 2006-01-12 US US11/795,043 patent/US20080193969A1/en not_active Abandoned
- 2006-01-12 WO PCT/NL2006/050006 patent/WO2006091094A2/en active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1384782A1 (en) * | 2001-03-30 | 2004-01-28 | National Institute of Advanced Industrial Science and Technology | Method of detecting the expression of aspergillus gene |
JP2003250590A (en) * | 2001-12-27 | 2003-09-09 | National Institute Of Advanced Industrial & Technology | Aspergillus oryzae flh2 gene |
EP1609867A1 (en) * | 2004-06-25 | 2005-12-28 | Feng Chia University | Nucleic acid construct, expression vector and method for enhancing the production of recombinant protein |
Non-Patent Citations (12)
Title |
---|
BHAVE SANJAY L ET AL: "Expression of Vitreoscilla hemoglobin improves growth and levels of extracellular enzyme in Yarrowia lipolytica.", BIOTECHNOLOGY AND BIOENGINEERING, vol. 84, no. 6, 20 December 2003 (2003-12-20), pages 658 - 666, XP002332564, ISSN: 0006-3592 * |
DATABASE EMBL [online] 1 March 2005 (2005-03-01), "Aspergillus oryzae hmb1 gene for putative haemoglobin", XP002403044, retrieved from EBI accession no. EM_PRO:AJ628839 Database accession no. AJ628839 * |
DATABASE EMBL [online] 28 July 2004 (2004-07-28), "Aspergillus fumigatus mRNA for putative flavohaemoglobin (fh gene)", XP002332571, retrieved from EBI accession no. EM_FUN:AFU578496 Database accession no. AFU578496 * |
DATABASE Geneseq [online] 28 March 2003 (2003-03-28), "Aspergillus oryzae polynucleotide SEQ ID NO 4117.", XP002332570, retrieved from EBI accession no. GSN:ABZ55004 Database accession no. ABZ55004 * |
DEMODENA J A ET AL: "The production of cephalosporin C by Acremonium chrysogenum is improved by the intracellular expression of a bacterial hemoglobin", BIO/TECHNOLOGY, NATURE PUBLISHING CO. NEW YORK, US, no. 11, August 1993 (1993-08-01), pages 926 - 929, XP002076831, ISSN: 0733-222X * |
FREY ALEXANDER D ET AL: "Bacterial hemoglobins and flavohemoglobins: Versatile proteins and their impact on microbiology and biotechnology.", FEMS MICROBIOLOGY REVIEWS, vol. 27, no. 4, October 2003 (2003-10-01), pages 525 - 545, XP002332565, ISSN: 0168-6445 * |
LIN YU-HUI ET AL: "Intracellular expression of Vitreoscilla hemoglobin in Aspergillus terreus to alleviate the effect of a short break in aeration during culture.", BIOTECHNOLOGY LETTERS, vol. 26, no. 13, July 2004 (2004-07-01), pages 1067 - 1072, XP002332563, ISSN: 0141-5492 * |
MIZUTANI OSAMU ET AL: "Disordered cell integrity signaling caused by disruption of the kexB gene in Aspergillus oryzae", EUKARYOTIC CELL, vol. 3, no. 4, August 2004 (2004-08-01), pages 1036 - 1048, XP002332567, ISSN: 1535-9778 * |
PUNT P J ET AL: "Filamentous fungi as cell factories for heterologous protein production", TRENDS IN BIOTECHNOLOGY, ELSEVIER PUBLICATIONS, CAMBRIDGE, GB, vol. 20, no. 5, 1 May 2002 (2002-05-01), pages 200 - 206, XP004347070, ISSN: 0167-7799 * |
TE BIESEBEKE R ET AL: "Different control mechanisms regulate glucoamylase and protease gene transcription in Aspergillus oryzae in solid-state and submerged fermentation.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. APR 2005, vol. 67, no. 1, April 2005 (2005-04-01), pages 75 - 82, XP002332568, ISSN: 0175-7598 * |
TE BIESEBEKE ROB: "Molecular analysis and improvement of protein production by Aspergillus oryzae grown on solid substrates", 29 March 2005, THESIS WAGENINGEN UNIVERSITY, WAGENINGEN, ISBN: 90-8504-144-9, XP002332569 * |
ZHU H ET AL: "YEAST FLAVOHEMOGLOBIN IS AN ANCIENT PROTEIN RELATED TO GLOBINS AND A REDUCTASE FAMILY", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 89, no. 11, 1992, pages 5015 - 5019, XP002332566, ISSN: 0027-8424 * |
Also Published As
Publication number | Publication date |
---|---|
WO2006091094A2 (en) | 2006-08-31 |
EP1841872A2 (en) | 2007-10-10 |
US20080193969A1 (en) | 2008-08-14 |
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