WO2003012098A1 - Levure modifiee presentant un potentiel secretoire eleve de proteine etrangere et procede permettant de produire une proteine etrangere a l'aide de la levure - Google Patents
Levure modifiee presentant un potentiel secretoire eleve de proteine etrangere et procede permettant de produire une proteine etrangere a l'aide de la levure Download PDFInfo
- Publication number
- WO2003012098A1 WO2003012098A1 PCT/JP2002/007857 JP0207857W WO03012098A1 WO 2003012098 A1 WO2003012098 A1 WO 2003012098A1 JP 0207857 W JP0207857 W JP 0207857W WO 03012098 A1 WO03012098 A1 WO 03012098A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- yeast
- foreign protein
- producing
- showing high
- high secretion
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/165—Yeast isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/72—Candida
Abstract
L'invention concerne un procédé permettant de produire une levure pouvant sécréter et produire un gène cible en grande quantité. La levure se caractérise par l'inactivation d'un gène codant pour sa principale protéine sécrétoire. Cette invention concerne également un procédé permettant de produire une protéine cible, consistant à cultiver ladite levure, laquelle à été transformée par un vecteur d'expression transportant un ADN codant pour la protéine cible, puis à recueillir la protéine cible dans le milieu de culture.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2001-233342 | 2001-08-01 | ||
JP2001233342A JP2003047471A (ja) | 2001-08-01 | 2001-08-01 | 異種タンパク質の分泌生産に優れた改変酵母およびその酵母を用いた異種タンパク質の製造法 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2003012098A1 true WO2003012098A1 (fr) | 2003-02-13 |
Family
ID=19065125
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2002/007857 WO2003012098A1 (fr) | 2001-08-01 | 2002-08-01 | Levure modifiee presentant un potentiel secretoire eleve de proteine etrangere et procede permettant de produire une proteine etrangere a l'aide de la levure |
Country Status (2)
Country | Link |
---|---|
JP (1) | JP2003047471A (fr) |
WO (1) | WO2003012098A1 (fr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011078351A1 (fr) * | 2009-12-25 | 2011-06-30 | キリンホールディングス株式会社 | Séquence de signal de sécrétion, et système d'expression de protéine utilisant celle-ci |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2009240185A (ja) * | 2008-03-28 | 2009-10-22 | Toyota Central R&D Labs Inc | 外来性タンパク質の高生産形質転換体及びその利用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995023857A1 (fr) * | 1994-03-05 | 1995-09-08 | Delta Biotechnology Limited | Souches de levure et albumines modifiees |
WO1998007873A1 (fr) * | 1996-08-23 | 1998-02-26 | Kirin Beer Kabushiki Kaisha | Vecteurs de levure et procede de production de proteines les utilisant |
WO1998014600A1 (fr) * | 1996-10-03 | 1998-04-09 | Centro De Ingenieria Genetica Y Biotecnologia (Cigb) | SYSTEME DE TRANSFORMATION DANS $i(CANDIDA UTILIS) |
WO2000014259A1 (fr) * | 1998-09-04 | 2000-03-16 | Kirin Beer Kabushiki Kaisha | Souches de candida boidinii presentant une activite protease reduite et utilisation de ces dernieres en tant qu'hote pour produire une proteine exogene |
-
2001
- 2001-08-01 JP JP2001233342A patent/JP2003047471A/ja active Pending
-
2002
- 2002-08-01 WO PCT/JP2002/007857 patent/WO2003012098A1/fr active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995023857A1 (fr) * | 1994-03-05 | 1995-09-08 | Delta Biotechnology Limited | Souches de levure et albumines modifiees |
WO1998007873A1 (fr) * | 1996-08-23 | 1998-02-26 | Kirin Beer Kabushiki Kaisha | Vecteurs de levure et procede de production de proteines les utilisant |
WO1998014600A1 (fr) * | 1996-10-03 | 1998-04-09 | Centro De Ingenieria Genetica Y Biotecnologia (Cigb) | SYSTEME DE TRANSFORMATION DANS $i(CANDIDA UTILIS) |
WO2000014259A1 (fr) * | 1998-09-04 | 2000-03-16 | Kirin Beer Kabushiki Kaisha | Souches de candida boidinii presentant une activite protease reduite et utilisation de ces dernieres en tant qu'hote pour produire une proteine exogene |
Non-Patent Citations (1)
Title |
---|
ROTHSTEIN RODNEY: "Targeting, disruption, replacement and allele rescue: integrative DNA transformation in yeast", METHODS IN ENZYMOLOGY, vol. 1974, 1991, pages 281 - 301, XP002958952 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011078351A1 (fr) * | 2009-12-25 | 2011-06-30 | キリンホールディングス株式会社 | Séquence de signal de sécrétion, et système d'expression de protéine utilisant celle-ci |
Also Published As
Publication number | Publication date |
---|---|
JP2003047471A (ja) | 2003-02-18 |
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