EP0578776A4 - Retrovirus inhibition with antisense nucleic acids complementary to packaging sequences. - Google Patents

Retrovirus inhibition with antisense nucleic acids complementary to packaging sequences.

Info

Publication number
EP0578776A4
EP0578776A4 EP92910993A EP92910993A EP0578776A4 EP 0578776 A4 EP0578776 A4 EP 0578776A4 EP 92910993 A EP92910993 A EP 92910993A EP 92910993 A EP92910993 A EP 92910993A EP 0578776 A4 EP0578776 A4 EP 0578776A4
Authority
EP
European Patent Office
Prior art keywords
retrovirus
inhibition
antisense nucleic
nucleic acids
acids complementary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP92910993A
Other languages
German (de)
French (fr)
Other versions
EP0578776A1 (en
Inventor
Thomas E Wagner
Lei Han
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EDISON ANIMAL BIOTECHNOLOGY CE
EDISON ANIMAL BIOTECH CENTER
Original Assignee
EDISON ANIMAL BIOTECHNOLOGY CE
EDISON ANIMAL BIOTECH CENTER
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EDISON ANIMAL BIOTECHNOLOGY CE, EDISON ANIMAL BIOTECH CENTER filed Critical EDISON ANIMAL BIOTECHNOLOGY CE
Publication of EP0578776A1 publication Critical patent/EP0578776A1/en
Publication of EP0578776A4 publication Critical patent/EP0578776A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0337—Animal models for infectious diseases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

Abstract

Antisense nucleic acid molecules are provided which hybridize essentially only to the packaging sequence of a retrovirus and thereby inhibit the packaging of the retroviral genomic RNA. The antisense molecules may be DNA, RNA, or analogues thereof. The antisense molecules may be administered as drugs, or the individual to be protected may be genetically manipulated to express an antisense RNA.
EP92910993A 1991-04-05 1992-04-03 Retrovirus inhibition with antisense nucleic acids complementary to packaging sequences. Withdrawn EP0578776A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US680873 1984-12-12
US68087391A 1991-04-05 1991-04-05

Publications (2)

Publication Number Publication Date
EP0578776A1 EP0578776A1 (en) 1994-01-19
EP0578776A4 true EP0578776A4 (en) 1995-04-12

Family

ID=24732881

Family Applications (1)

Application Number Title Priority Date Filing Date
EP92910993A Withdrawn EP0578776A4 (en) 1991-04-05 1992-04-03 Retrovirus inhibition with antisense nucleic acids complementary to packaging sequences.

Country Status (5)

Country Link
EP (1) EP0578776A4 (en)
JP (1) JPH06506599A (en)
AU (1) AU655279B2 (en)
CA (1) CA2107789A1 (en)
WO (1) WO1992017211A1 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4126484A1 (en) * 1991-08-10 1993-02-11 Bayer Ag Anti-sense-RNA expression vectors - contain hybrid promoter sequences and viral DNA sequences i.e. HIV, in anti-sense direction, useful in prodn. of HIV-resistant cells
DE4225094A1 (en) * 1992-04-28 1993-11-04 Frank Andreas Harald Meyer Compsn. for gene therapy, esp. of viral infections and tumours - comprises anti-sense DNA or RNA inserted into long terminal repeat or transposable element and e.g. virus envelope as carrier
HU219304B (en) 1992-09-25 2001-03-28 Inst Nat Sante Rech Med Recombinant adenovirus derived vectors for the transfer of foreign genes into cells of the central nervous system, pharmaceutical preparations containing them and processes for their production
EP0598935A1 (en) * 1992-11-24 1994-06-01 Bayer Ag Expression vectors and their use to produce HIV-resistant human cells for therapeutic use
US5583035A (en) * 1992-12-07 1996-12-10 Bayer Aktiengesellschaft HIV antisense expression vectors
FR2702152B1 (en) * 1993-03-03 1995-05-24 Inst Nat Sante Rech Med Recombinant viruses and their use in gene therapy.
US5712384A (en) * 1994-01-05 1998-01-27 Gene Shears Pty Ltd. Ribozymes targeting retroviral packaging sequence expression constructs and recombinant retroviruses containing such constructs
ATE320487T1 (en) * 1994-01-05 2006-04-15 Gene Shears Pty Ltd RIBOZYMES AGAINST AN HIV ACT SEQUENCE
KR100696410B1 (en) * 1994-01-05 2008-11-07 진 쉬어즈 피티와이., 엘티디. Ribozymes and Retrocombination Retroviruses Having Retroviral Packaging Sequence Expression Constructs
AU2133695A (en) * 1994-04-06 1995-10-30 Sadna Joshi-Sukhwal Inhibition of hiv-1 multiplication in mammalian cells
US20020058636A1 (en) * 1994-09-21 2002-05-16 Geoffrey P. Symonds Ribozymes targeting the retroviral packaging sequence expression constructs and recombinant retroviruses containing such constructs
ES2358187T3 (en) 2001-07-10 2011-05-06 JOHNSON & JOHNSON RESEARCH PTY LIMITED PROCEDURES FOR THE GENETIC MODIFICATION OF HEMATOPOYETIC PROGENITING CELLS AND USES OF THE MODIFIED CELLS.
BR0211079A (en) 2001-07-10 2005-08-30 Johnson & Johnson Res Pty Ltd Processes for genetic modification of progenitor hematopoietic cells and use of modified cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4740463A (en) * 1984-04-13 1988-04-26 Massachusetts Institute Of Technology Methods and artificial genes for antagonizing the function of an oncogene
WO1988010311A1 (en) * 1987-06-26 1988-12-29 Syntro Corporation Recombinant human cytomegalovirus containing foreign gene and use thereof
WO1989008146A1 (en) * 1988-02-26 1989-09-08 Worcester Foundation For Experimental Biology Inhibition of htlv-iii by exogenous oligonucleotides
EP0331939A2 (en) * 1988-02-16 1989-09-13 Greatbatch Gen-Aid, Ltd Method and compositions for conferring resistance to retroviral infection

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4757055A (en) * 1980-11-12 1988-07-12 The Johns Hopkins University Method for selectively controlling unwanted expression or function of foreign nucleic acids in animal or mammalian cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4740463A (en) * 1984-04-13 1988-04-26 Massachusetts Institute Of Technology Methods and artificial genes for antagonizing the function of an oncogene
WO1988010311A1 (en) * 1987-06-26 1988-12-29 Syntro Corporation Recombinant human cytomegalovirus containing foreign gene and use thereof
EP0331939A2 (en) * 1988-02-16 1989-09-13 Greatbatch Gen-Aid, Ltd Method and compositions for conferring resistance to retroviral infection
WO1989008146A1 (en) * 1988-02-26 1989-09-08 Worcester Foundation For Experimental Biology Inhibition of htlv-iii by exogenous oligonucleotides

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HAN L ET AL: "INHIBITION OF MOLONEY MURINE LEUKEMIA VIRUS-INDUCED LEUKEMIA IN TRANSGENIC MICE EXPRESSING ANTISENSE RNA COMPLEMENTARY TO THE RETROVIRAL PACKAGING SEQUENCES.", PROC NATL ACAD SCI U S A 88 (10). 1991. 4313-4317. *
SANFORD, J.: "Applying the PDR principle to AIDS", J. THEOR. BIOL., vol. 130, 1988, pages 469 - 480 *
See also references of WO9217211A1 *

Also Published As

Publication number Publication date
AU655279B2 (en) 1994-12-15
JPH06506599A (en) 1994-07-28
EP0578776A1 (en) 1994-01-19
WO1992017211A1 (en) 1992-10-15
AU1778092A (en) 1992-11-02
CA2107789A1 (en) 1992-10-06

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